Burger D R, Vandenbark A A, Daves D, Anderson W A, Vetto R M, Finke P
J Immunol. 1976 Sep;117(3):789-96.
Human transfer factor (TF) was fractionated by exclusion chromatography and the fractions were tested for biologic activity in vivo and in vitro. Specific TF activity in vivo was found to reside in the major UV-absorbing peak (Fraction III). Fraction III eluted at 2.7 X V(O) and transferred tuberculin, candida, or KLH-reactivity to previously negative recipients. Fraction III from nonreactive donors was ineffective. When the fractions were tested in vitro, we found that both the mitogenic activity of whole TF and the suppressive activity to mitogen activation when present in TF was found in Fraction I. Fraction III contained components responsible for augmentation of PHA and PWM responses. In addition, Fraction III contained the component responsible for antigen-dependent augmentation of lymphocyte transformation. Fraction IV was suppressive to antigen-induced lymphocyte transformation. These data suggest that TF preparations contain components which can affect immune reactions in both specific and nonspecific ways.
人转移因子（TF）通过排阻色谱法进行分级分离，然后对各组分进行体内和体外生物活性测试。发现体内特异性TF活性存在于主要的紫外线吸收峰（组分III）中。组分III在2.7×V（O）处洗脱，并将结核菌素、念珠菌或钥孔戚血蓝蛋白反应性转移给先前无反应的受体。来自无反应供体的组分III无效。当在体外对各组分进行测试时，我们发现TF整体的促有丝分裂活性以及TF中存在时对有丝分裂原激活的抑制活性均存在于组分I中。组分III含有负责增强PHA和PWM反应的成分。此外，组分III含有负责抗原依赖性增强淋巴细胞转化的成分。组分IV对抗原诱导的淋巴细胞转化具有抑制作用。这些数据表明，TF制剂含有能够以特异性和非特异性方式影响免疫反应的成分。
