Hsu J W, Hsu S L, Chu J J, Liu M C, Chiang C D
Department of Internal Medicine, Taichung Veterans General Hospital, Taiwan.
Anticancer Res. 1997 Jan-Feb;17(1A):407-11.
The nm23 and mts1 genes have been the focus of attention as regards the association of their expression with metastatic behaviour. The level of nm23 and mts1 gene products has been demonstrated to correlate with metastatic potential in some tumors, but not in all. Here we show that these two genes might be coregulated and the ratio of their expression correlated with metastatic behaviour. Western blot analysis showed that the expression of both NM23 and MTS1 proteins was reduced in human lung cancer CH27 cells by retinoic acid treatment, but the ratio of NM23: MTS1 increased in a dose-dependent manner. Results also exhibited that retinoic acid altered the microtubule assembly of CH27 cells and reduced the metastatic ability of the cells in vitro. These data suggest that the metastatic potential of CH27 cells may be related to the relative expression of these two genes, and that their pathway in regulating metastatsis might be linked.
nm23和mts1基因一直是其表达与转移行为关联方面的关注焦点。nm23和mts1基因产物的水平已被证明在某些肿瘤中与转移潜能相关，但并非在所有肿瘤中都如此。在此我们表明这两个基因可能受到共同调控，且它们的表达比例与转移行为相关。蛋白质印迹分析显示，用视黄酸处理后人肺癌CH27细胞中NM23和MTS1蛋白的表达均降低，但NM23:MTS1的比例呈剂量依赖性增加。结果还表明视黄酸改变了CH27细胞的微管组装，并降低了细胞在体外的转移能力。这些数据表明CH27细胞的转移潜能可能与这两个基因的相对表达有关，并且它们在调节转移中的途径可能是相关联的。
