Décimo D, Boespflug O, Meunier-Rotival M, Hadchouel M, Tardieu M
Laboratoire de Neurovirologie et de Neuroimmunologie, Université Paris XI, France.
Arch Virol. 1993;130(3-4):269-77. doi: 10.1007/BF01309659.
To study the host-dependent genetic variations in murine hepatitis virus type 3 (MHV 3) induced diseases, we localized the sites of MHV 3 (Mill Hill strain) expression within liver and brain by immunohistochemistry or hybridization in situ. Two strains of mice were studied: BALB/c mice, which develop an acute and lethal hepatitis and C3H mice which develop a chronic brain infection. In BALB/c mice, viral RNA and antigens appeared during the first 24h post infection (p.i.) in liver, whereas viral RNA was barely detectable in brain, up until death at day 3 p.i. In C3H mice, viral RNA and antigens were detected simultaneously in liver and brain only at day 2 p.i. In brain, the virus was detected in meningeal and ependymal cells and in perivascular cortical areas (days 5 and 7 p.i.). After day 49, the virus was no longer detected in brain parenchyma, but persisted in meningeal cells. Two host-dependent genetic differences in viral processing were observed in the liver: (1) the virus was first detected in Kupffer cells in BALB/c mice and mostly in hepatocytes in C3H mice; (2) in BALB/c mice, the 180 kDa S viral glycoprotein appeared more frequently cleaved in 90 kDa form than in C3H mice.
为研究3型鼠肝炎病毒（MHV 3）诱导疾病中宿主依赖性的基因变异，我们通过免疫组织化学或原位杂交确定了肝脏和脑内MHV 3（米尔希尔毒株）的表达位点。研究了两种品系的小鼠：BALB/c小鼠，会发生急性致死性肝炎；C3H小鼠，会发生慢性脑部感染。在BALB/c小鼠中，感染后（p.i.）最初24小时内肝脏中出现病毒RNA和抗原，而在脑内直到感染后第3天死亡时病毒RNA才勉强可检测到。在C3H小鼠中，仅在感染后第2天在肝脏和脑内同时检测到病毒RNA和抗原。在脑内，病毒在脑膜和室管膜细胞以及血管周围皮质区域被检测到（感染后第5天和第7天）。49天后，在脑实质中不再检测到病毒，但在脑膜细胞中持续存在。在肝脏中观察到病毒处理过程中有两种宿主依赖性基因差异：（1）在BALB/c小鼠中病毒首先在库普弗细胞中被检测到，而在C3H小鼠中大多在肝细胞中被检测到；（2）在BALB/c小鼠中，180 kDa的S病毒糖蛋白比在C3H小鼠中更频繁地裂解为90 kDa形式。
