Devictor B, Bonnier P, Piana L, Andrac L, Lavaut M N, Allasia C, Charpin C
DRED Unit (Human solid tumor oncogenesis), Centre Hospitalo-Universitaire Timone, Marseille, France.
Gynecol Oncol. 1993 Jun;49(3):284-90. doi: 10.1006/gyno.1993.1128.
Cone biopsies were carried out in patients (n = 72) with intraepithelial lesions (CIN 1-3) and squamous microinvasive carcinomas of the uterine cervix. Intraoperative histological examination was achieved in order to obtain small cones with healthy upper resection margins. Also, a tissue fragment was sampled and frozen for the immunocytochemical investigations. Image analysis (SAMBA) of immunoprecipitates was used to evaluate c-myc and Ki-67 antigen expression. Positive c-myc protein staining was observed only on microinvasive carcinomas (70%) and CIN 3 lesions (19%) and was correlated with high Ki-67 immunoreactivity. Ki-67 immunostaining was more extensive: (i) in high-grade than low-grade CIN, (ii) in microinvasive carcinomas than in CIN (P < or = 0.01), (iii) in large than in small lesions. No correlation could be found between the levels of either c-myc protein or Ki-67 antigen expression and either patient age or cytological features of HPV infection.
对患有宫颈上皮内病变（CIN 1 - 3）和宫颈鳞状微浸润癌的患者（n = 72）进行了锥形活检。为了获取上切缘健康的小锥体，进行了术中组织学检查。此外，采集了组织碎片并冷冻用于免疫细胞化学研究。使用免疫沉淀物的图像分析（SAMBA）来评估c - myc和Ki - 67抗原的表达。仅在微浸润癌（70%）和CIN 3病变（19%）中观察到c - myc蛋白阳性染色，且与高Ki - 67免疫反应性相关。Ki - 67免疫染色更广泛：（i）在高级别CIN中比在低级别CIN中更广泛，（ii）在微浸润癌中比在CIN中更广泛（P≤0.01），（iii）在大病变中比在小病变中更广泛。在c - myc蛋白或Ki - 67抗原表达水平与患者年龄或HPV感染的细胞学特征之间未发现相关性。
