Shoji Y, Saegusa M, Takano Y, Ohbu M, Okayasu I
Department of Pathology, Kitasato University School of Medicine, Kanagawa, Japan.
J Clin Pathol. 1996 Feb;49(2):134-8. doi: 10.1136/jcp.49.2.134.
To clarify the significance of apoptosis in the progression of uterine cervical neoplasias, including cervical intraepithelial neoplasia (CIN), microinvasive carcinoma (MIC), and invasive squamous cell carcinoma (ISCC) categories, in relation to cell proliferation and human papilloma virus (HPV) infection.
Forty six cases of CIN I/II, 75 of CIN III, 16 of MIC, and 44 of ISCC were examined using formalin fixed and paraffin wax embedded samples. The TdT mediated dUTP-biotin nick end labelling (TUNEL) method for detection of apoptotic cells was performed along with Ki-67 immunohistochemistry. Presence of HPV-DNA was confirmed by PCR-RFLP assay.
Apoptotic labelling indices, calculated after counting positive nuclei among at least 2000 nuclei, showed significant positive correlation with histological malignant grading in CIN and tumour cell invasion into stroma. In contrast, similar Ki-67 labelling index values were found in CIN, MIC, and ISCC. Although HPV-DNA was detected in 35/46 CIN I/II (76.1%), 53/74CIN III (71.6%), 9/16 MIC (56.3%), and 36/44 ISCC (81.8%), there was no apparent relation with the apoptotic labelling indices.
Apoptosis in cervical neoplasias may be closely related to tumour cell differentiation and progression. It also seems unlikely that HPV itself is directly related to pathways regulating apoptosis.
阐明凋亡在子宫颈肿瘤进展中的意义，这些肿瘤包括宫颈上皮内瘤变（CIN）、微浸润癌（MIC）和浸润性鳞状细胞癌（ISCC），并探讨其与细胞增殖和人乳头瘤病毒（HPV）感染的关系。
采用福尔马林固定、石蜡包埋的样本，对46例CIN I/II、75例CIN III、16例MIC和44例ISCC进行检测。采用TdT介导的dUTP生物素缺口末端标记（TUNEL）法检测凋亡细胞，并进行Ki-67免疫组化。通过PCR-RFLP分析确认HPV-DNA的存在。
在至少2000个细胞核中计数阳性细胞核后计算的凋亡标记指数，与CIN中的组织学恶性分级和肿瘤细胞向基质的浸润呈显著正相关。相比之下，CIN、MIC和ISCC中的Ki-67标记指数值相似。虽然在35/46例CIN I/II（76.1%）、53/74例CIN III（71.6%）、9/16例MIC（56.3%）和36/44例ISCC（81.8%）中检测到HPV-DNA，但与凋亡标记指数无明显关系。
宫颈肿瘤中的凋亡可能与肿瘤细胞分化和进展密切相关。HPV本身似乎也不太可能直接参与调节凋亡的途径。
