Powell A, Sloss A M, Smith R N, Murrell H
Cancer Res. 1979 Feb;39(2 Pt 2):570-5.
The hemacytometer leukocyte adherence inhibition (LAI) assay was investigated with respect to immunological relevance, specificity, and cellular mechanisms. Humans were immunized to keyhole limpet hemocyanin, and rats were immunized to dinitrophenyl-bovine gamma-globulin. LAI analysis disclosed classic patterns of immune response kinetics. The LAI response was dose dependent in vitro with no inhibition at relatively high antigen doses. In vitro specificity in rats was restricted to the immunizing conjugate. Cells forming spontaneous E-rosettes were required for LAI reactions. Lymphokine production required the presence of E-rosette-forming cells. E-rosette-forming cells from normal donors lost adherence in the presence of lymphokine. The requirement for T-lymphocytes was confirmed in a human osteosarcoma system using independent criteria. Thus, the hemacytometer LAI depends upon T-lymphocyte collaboration via a lymphokine. It should be distinguished from the tube and microplate variants of LAI analysis because these appear to depend upon different mechanisms.
对血细胞计数器白细胞黏附抑制（LAI）试验进行了免疫相关性、特异性和细胞机制方面的研究。人类用钥孔戚血蓝蛋白进行免疫，大鼠用二硝基苯基 - 牛γ球蛋白进行免疫。LAI分析揭示了免疫反应动力学的经典模式。LAI反应在体外呈剂量依赖性，在相对高的抗原剂量下无抑制作用。大鼠体外的特异性仅限于免疫结合物。LAI反应需要形成自发E花环的细胞。淋巴因子的产生需要有E花环形成细胞。来自正常供体的E花环形成细胞在淋巴因子存在时失去黏附性。在人骨肉瘤系统中使用独立标准证实了对T淋巴细胞的需求。因此，血细胞计数器LAI依赖于通过淋巴因子的T淋巴细胞协作。它应与LAI分析的试管和微孔板变体区分开来，因为这些似乎依赖于不同的机制。
