Ziegenhorn J, Neumann U, Hagen A, Bablok W, Strinshoff K
J Clin Chem Clin Biochem. 1977 Jan;15(1):13-9. doi: 10.1515/cclm.1977.15.1-12.13.
Studies are reported on the reaction kinetics of the glucose assay according to Trinder which involves the specific oxidation of glucose by glucose oxidase and the determination of the hydrogen peroxide released by means of phenol and 4-aminophenazone in the presence of peroxidase. The results have been used to develop a general kinetic fixed-time method for the analysis of glucose in whole blood and serum. The single reagent method has been adapted to the ENI GEMSAEC centrifugal analyzer and to the Abbott ABA-100 analyzer. The procedures exhibited excellent precision and the results correlated well with those obtained by the hexokinase method, Linearity was achieved from 3 to 64 mmol/1 glucose for the GEMSAEC method, and from 3 to 33 mmol/1 glucose for the ABA-100 method. Reagent or sample blank corrections were not necessary. There were no interferences from various drugs, hemoglobin, bilirubin, or lipemia.
本文报道了根据Trinder法进行葡萄糖检测的反应动力学研究，该方法涉及葡萄糖氧化酶对葡萄糖的特异性氧化，以及在过氧化物酶存在的情况下，通过苯酚和4-氨基苯腙测定释放的过氧化氢。研究结果已用于开发一种通用的动力学固定时间法，用于分析全血和血清中的葡萄糖。单试剂法已适用于ENI GEMSAEC离心分析仪和雅培ABA-100分析仪。该方法具有出色的精密度，结果与己糖激酶法所得结果相关性良好。GEMSAEC法在3至64 mmol/1葡萄糖范围内实现线性，ABA-100法在3至33 mmol/1葡萄糖范围内实现线性。无需进行试剂或样品空白校正。各种药物、血红蛋白、胆红素或脂血均无干扰。
