Xie T X
Institute of Basic Medicine, Hebei Medical College, Shijiazhuang.
Zhonghua Yi Xue Za Zhi. 1993 Feb;73(2):78-80, 125-6.
To detect the carcinogenicity of sterigmatocystin for human stomach, the human fetal gastric cells, cultured in vitro, were treated with extract solution of Aspergillus versicolor culture (0.117mg sterigmatocystin per Kg culture of Aspergillus versicolor). The cells showed random arrangement, loss of contact inhibition and cell polarity. Some transformed foci were present in different numbers and times according to the dosages of the extract solution. The cells with such changes were analysed with a flow cytometer and the results showed that the cell number in S phase of cell cycle distribution was greatly increased. The above changes were similar to those of the cells treated with MNNG, but no such changes were noted in the control group. These results indicated that sterigmatocystin could induce some characteristics of neoplastic transformed cells and that sterigmatocystin may be a carcinogen for human gastric cancer.
为检测杂色曲霉素对人胃的致癌性，将体外培养的人胎儿胃细胞用杂色曲霉培养物提取液（每千克杂色曲霉培养物含0.117毫克杂色曲霉素）处理。细胞呈现排列紊乱、接触抑制丧失和细胞极性消失。根据提取液剂量不同，在不同时间出现了数量不等的转化灶。用流式细胞仪对发生上述变化的细胞进行分析，结果显示细胞周期分布中S期的细胞数量大幅增加。上述变化与用N-甲基-N'-硝基-N-亚硝基胍处理的细胞相似，但对照组未观察到此类变化。这些结果表明，杂色曲霉素可诱导肿瘤转化细胞的某些特征，杂色曲霉素可能是人类胃癌的致癌物。
