Matsuoka I, Mori T, Aoki J, Sato T, Kurihara K
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Biochem Biophys Res Commun. 1993 Jul 15;194(1):504-11. doi: 10.1006/bbrc.1993.1848.
Using reverse transcription-polymerase chain reaction (RT-PCR) and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Physiological functions of these receptors in taste cells are discussed.
利用逆转录-聚合酶链反应（RT-PCR）和简并寡核苷酸引物，我们从牛味觉组织中扩增出了G蛋白偶联受体（GCR）超家族两个不同亚家族的新成员。由多个cDNA克隆组成的A型受体克隆与假定的嗅觉受体亚家族有显著相似性，而单个B型克隆与肽受体亚家族有显著相似性。文中讨论了这些受体在味觉细胞中的生理功能。
