粒细胞巨噬细胞集落刺激因子可刺激人髓系来源细胞中磷酸肌醇3-OH激酶和src相关酪氨酸激酶的结合与激活。
Granulocyte macrophage-colony stimulating factor stimulates both association and activation of phosphoinositide 3OH-kinase and src-related tyrosine kinase(s) in human myeloid derived cells.
作者信息
Corey S, Eguinoa A, Puyana-Theall K, Bolen J B, Cantley L, Mollinedo F, Jackson T R, Hawkins P T, Stephens L R
机构信息
Department of Pediatrics, University of Pittsburgh, PA 15213.
出版信息
EMBO J. 1993 Jul;12(7):2681-90. doi: 10.1002/j.1460-2075.1993.tb05929.x.
The signalling pathways used by the GM-CSF receptor are currently unknown. Here we show that in human myeloid derived cells GM-CSF can stimulate; (i) the accumulation of PtdIns(3,4,5)P3; (ii) increases in p53/p56lyn and p62c-yes directed protein tyrosine kinase activities in anti-lyn and anti-c-yes antibody directed immunoprecipitates, respectively and; (iii) increases in phosphoinositide 3OH-kinase activity in antiphosphotyrosine, anti-p53/p56lyn and anti-p62c-yes antibody directed immunoprecipitates. These results suggest that GM-CSF can stimulate formation of protein tyrosine kinase co-ordinated signalling complexes, that contain p53/p56lyn, p62c-yes and an activated PtdInsP2 directed phosphoinositide 3OH-kinase, which can drive the accumulation of the putative second-messenger PtdIns(3,4,5)P3.
目前尚不清楚GM-CSF受体所使用的信号通路。在此我们表明，在人骨髓来源的细胞中，GM-CSF可刺激：（i）磷脂酰肌醇-3,4,5-三磷酸（PtdIns(3,4,5)P3）的积累；（ii）分别在抗Lyn和抗c-Yes抗体介导的免疫沉淀物中，p53/p56Lyn和p62c-Yes定向蛋白酪氨酸激酶活性增加；以及（iii）在抗磷酸酪氨酸、抗p53/p56Lyn和抗p62c-Yes抗体介导的免疫沉淀物中，磷酸肌醇3-羟基激酶活性增加。这些结果表明，GM-CSF可刺激蛋白酪氨酸激酶协调信号复合物的形成，该复合物包含p53/p56Lyn、p62c-Yes和一种活化的磷脂酰肌醇-2（PtdInsP2）定向磷酸肌醇3-羟基激酶，其可驱动假定的第二信使PtdIns(3,4,5)P3的积累。
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