Donta S T, Beristain S, Tomicic T K
Department of Medicine, University of Connecticut Health Center, Farmington 06030.
Infect Immun. 1993 Aug;61(8):3282-6. doi: 10.1128/iai.61.8.3282-3286.1993.
Cholera enterotoxin and the related heat-labile enterotoxins of Escherichia coli enter their target cells through noncoated vesicles, but how the toxins are processed intracellularly and how they get to their targeted enzyme, adenylate cyclase, remain to be defined. Brefeldin A, an inhibitor of the trans-Golgi network, is shown herein to transiently block the morphologic and enzymatic effects of the toxin at a step distal to the initial binding process but prior to activation of adenylate cyclase by the toxin. It is likely, therefore, that these toxins are processed by the Golgi apparatus before trafficking to the membrane adenylate cyclase.
霍乱肠毒素及大肠杆菌相关的不耐热肠毒素通过无被小泡进入其靶细胞，但毒素在细胞内如何被加工处理以及如何到达其靶标酶——腺苷酸环化酶，仍有待确定。本文显示，反式高尔基体网络的抑制剂布雷菲德菌素A在毒素初始结合过程之后但在毒素激活腺苷酸环化酶之前的一个步骤中，能短暂阻断毒素的形态学和酶学效应。因此，这些毒素很可能在转运至膜结合的腺苷酸环化酶之前由高尔基体进行加工处理。
