Smíd B, Valícek L, Rodák L, Kudrna J, Musilová J
Výzkumný ústav veterinárního lékaŕství, Brno.
Vet Med (Praha). 1993;38(6):333-41.
Coronavirus-induced porcine epidemic diarrhoea (PED) was diagnosed in two swine herds. The causal agent was demonstrated in intestinal contents by electron microscopy and identified by immunoelectron microscopy using specific immune serum to the reference strain PED-CV77. Experimental transmission to hysterectomy-derived, colostrum-deprived piglets with an intestinal contents filtrate was successful. The virus was demonstrable by electron microscopy in the intestinal contents between 12th hour and 4th day, and in small intestinal epithelial cells 18 hours after infection. Scanning electron microscopy revealed shortening and fusion of villi of small intestinal mucosa.
在两个猪群中诊断出冠状病毒引起的猪流行性腹泻（PED）。通过电子显微镜在肠道内容物中证实了病原体，并使用针对参考菌株PED-CV77的特异性免疫血清通过免疫电子显微镜进行了鉴定。用肠道内容物滤液对子宫切除、初乳缺乏的仔猪进行实验性传播获得成功。在感染后12小时至第4天之间，通过电子显微镜在肠道内容物中可检测到该病毒，在感染后18小时在小肠上皮细胞中也可检测到。扫描电子显微镜显示小肠黏膜绒毛缩短和融合。
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