一种针对粪便中猪流行性腹泻病毒检测进行优化的酶联免疫吸附测定法。
An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces.
作者信息
Rodák L, Valícek L, Smíd B, Nevoránková Z
机构信息
Veterinary Research Institute, Department of Virology, Hudcova 70, 621 32 Brno, Czech Republic.
出版信息
Vet Microbiol. 2005 Jan 5;105(1):9-17. doi: 10.1016/j.vetmic.2004.09.020. Epub 2004 Nov 23.
Monoclonal antibodies to porcine epidemic diarrhoea virus (PEDV) membrane protein M were prepared and used for the comparative assessment of three blocking ELISA variants to detect PEDV. The competitive blocking ELISA (CB-ELISA) format showed the highest sensitivity, allowing detection of 10(2.5) plaque-forming units of PEDV/ml in culture medium. Its specificity was verified by inclusion of control samples containing transmissible gastroenteritis virus (TGEV) and rotavirus A in each analysis. Eighty porcine field samples of faeces obtained from 38 herds affected with diarrhoea were examined, and PEDV was found in 15 (19%) samples from 6 (16%) herds. The suitability of the CB-ELISA for the screening herds in epizootiologic situations is discussed.
制备了针对猪流行性腹泻病毒（PEDV）膜蛋白M的单克隆抗体，并用于比较评估三种阻断ELISA变体以检测PEDV。竞争阻断ELISA（CB-ELISA）形式显示出最高的灵敏度，能够检测培养基中10(2.5)个PEDV/ml的蚀斑形成单位。通过在每次分析中加入含有传染性胃肠炎病毒（TGEV）和轮状病毒A的对照样品来验证其特异性。对从38个腹泻感染猪群中采集的80份猪粪便现场样品进行了检测，在6个（16%）猪群的15份（19%）样品中发现了PEDV。讨论了CB-ELISA在动物流行病学情况下用于筛查猪群的适用性。
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