Yoshida N, Majima T, Yamaguchi M, Horie T
First Department of Internal Medicine, Nihon University School of Medicine.
Arerugi. 1993 Jun;42(6):714-22.
To investigate the interaction of arachidonic acid metabolites, we examined the production of LTB4 in neutrophils after stimulation with PGE2, PGF2 alpha and TXB2. The subjects were 23 asthmatic patients (atopic: 11, non-atopic: 12), 7 non-asthmatic atopic patients and 5 healthy controls. The production of LTB4 in neutrophils induced by calcium ionophore A23187 significantly increased in the asthmatic and non-asthmatic atopic patients. Healthy controls showed no significant increase in production of LTB4. The optimal levels of TXB2, PGF2 alpha, and PGE2 for LTB4 production were 10 ng/ml, 10 ng/ml and 0.1 ng/ml, respectively. When no TXB2, PGF2 alpha and PGE2 were added, LTB4 production tended to be greater in the asthmatics and non-asthmatic atopic patients than in the controls. By the addition of 10 ng/ml of TXB2, 10 ng/ml of PGF2 alpha, or 0.1 ng/ml PGE2, production of LTB4 by calcium ionophore A23187 stimulation was significantly increased in the asthmatics, and there was no such trend in the non-asthmatic group. These results suggest that LTB4 production from neutrophils may be enhanced by TXB2, PGF2 alpha and PGE2.
为了研究花生四烯酸代谢产物的相互作用，我们检测了用前列腺素E2（PGE2）、前列腺素F2α（PGF2α）和血栓素B2（TXB2）刺激后中性粒细胞中白三烯B4（LTB4）的产生。研究对象包括23例哮喘患者（特应性：11例，非特应性：12例）、7例非哮喘特应性患者和5名健康对照者。钙离子载体A23187诱导的中性粒细胞中LTB4的产生在哮喘患者和非哮喘特应性患者中显著增加。健康对照者的LTB4产生无显著增加。产生LTB4的TXB2、PGF2α和PGE2的最佳水平分别为10 ng/ml、10 ng/ml和0.1 ng/ml。当不添加TXB2、PGF2α和PGE2时，哮喘患者和非哮喘特应性患者的LTB4产生往往高于对照组。通过添加10 ng/ml的TXB2、10 ng/ml的PGF2α或0.1 ng/ml PGE2，钙离子载体A23187刺激后哮喘患者的LTB4产生显著增加，而非哮喘组则无此趋势。这些结果表明，TXB2、PGF2α和PGE可增强中性粒细胞产生LTB4。
