Roubin R, Elsas P P, Fiers W, Dessein A J
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
Clin Exp Immunol. 1987 Nov;70(2):484-90.
Recombinant human tumour necrosis factor alpha (rTNF) did not cause the release of leukotrienes (LT) by human neutrophils and eosinophils. It did, however, prepare neutrophils and eosinophils for enhanced production of LT after activation with the calcium ionophore A23187. The effect was observed with 10(2) to 10(4) units/ml of rTNF which enhanced LTB4 production by ionophore-stimulated neutrophils, by 40 to 400%, while 10(3) to 10(4) units/ml of rTNF increased LTC4 production by ionophore-stimulated eosinophils by 30 to 120%. The enhancement was observed when granulocytes were incubated for 5 to 10 min with rTNF before stimulation with the ionophore. Increased leukotriene production was attributed to the induction of fatty acid hydrolase and/or 5-lipoxygenase activities on the basis of the increment in the total counts released after 3H-arachidonic acid labelling of neutrophils pretreated with rTNF as compared to control medium.
重组人肿瘤坏死因子α（rTNF）不会使人中性粒细胞和嗜酸性粒细胞释放白三烯（LT）。然而，它确实使中性粒细胞和嗜酸性粒细胞在经钙离子载体A23187激活后，白三烯的生成量增加。在10²至10⁴单位/毫升的rTNF作用下可观察到这种效应，该浓度范围的rTNF可使离子载体刺激的中性粒细胞产生的白三烯B4增加40%至400%，而10³至10⁴单位/毫升的rTNF可使离子载体刺激的嗜酸性粒细胞产生的白三烯C4增加30%至120%。当粒细胞在用离子载体刺激前与rTNF孵育5至10分钟时，可观察到这种增强作用。与对照培养基相比，在用rTNF预处理的中性粒细胞经³H - 花生四烯酸标记后，释放的总数增加，基于此，白三烯生成量的增加归因于脂肪酸水解酶和/或5 - 脂氧合酶活性的诱导。
