Shaheen A A, Hamdy M A, Kheir-Eldin A A, Lindström P, el-Fattah A A
Department of Biochemistry, Faculty of Pharmacy, Cairo University, Egypt.
Biochem Pharmacol. 1993 Jul 6;46(1):194-7. doi: 10.1016/0006-2952(93)90367-6.
The effect of vitamin E (VE) or diazepam (DZ) pretreatment on some carbohydrate metabolic aspects in the brains of stressed rats was studied. DZ and VE were given i.p. at doses of 5 mg/kg body wt for 6 days prior to subjecting the animals to single swimming stress (SSS). Pretreatment of the rats with DZ or VE diminished the stress-induced increases in plasma corticosterone and glucose levels and reversed the decrease due to stress on brain ATP, glucose, glycogen and pyruvate contents. The increase in brain ADP and lactate was brought back to levels which approached the pre-stressed values. Moreover, DZ and VE pretreatments helped in attenuating the stress-induced alteration in brain mitochondrial and cytosolic hexokinase as well as sodium, potassium adenosine triphosphatase (Na+,K(+)-ATPase) activities. The change in these metabolic parameters produced by VE pre-treatment was less than that exhibited by DZ. The effects of VE were explained in light of its antioxidant property in preventing the free radical production and lipid peroxide formation which are important factors in the pathogenesis of stress.
研究了维生素E（VE）或地西泮（DZ）预处理对应激大鼠大脑中某些碳水化合物代谢方面的影响。在使动物遭受单次游泳应激（SSS）前6天，以5mg/kg体重的剂量腹腔注射DZ和VE。用DZ或VE预处理大鼠可减轻应激诱导的血浆皮质酮和葡萄糖水平升高，并逆转应激导致的大脑ATP、葡萄糖、糖原和丙酮酸含量降低。大脑ADP和乳酸的增加恢复到接近应激前值的水平。此外，DZ和VE预处理有助于减轻应激诱导的大脑线粒体和胞质己糖激酶以及钠钾三磷酸腺苷酶（Na +，K（+）-ATPase）活性的改变。VE预处理产生的这些代谢参数变化小于DZ表现出的变化。根据VE的抗氧化特性来解释其作用，即预防自由基产生和脂质过氧化物形成，而这两者是应激发病机制中的重要因素。
