Jaiswal Sunil Kumar, Siddiqi Nikhat Jamal, Sharma Bechan
Department of Biochemistry, University of Allahabad, Allahabad, 211002, India.
J Biochem Mol Toxicol. 2014 Jul;28(7):320-7. doi: 10.1002/jbt.21568. Epub 2014 May 27.
Pesticides cause oxidative stress and adversely influence Na(+)-K(+)-ATPase activity in animals. Since impact of carbofuran has not been properly studied in the mammalian brain, the ability of carbofuran to induce oxidative stress and modulation in Na(+)-K(+)-ATPase activity and its amelioration by vitamin E was performed. The rats divided into six groups received two different doses of carbofuran (15% and 30% LD50) for 15 days. The results suggested that the carbofuran treatment caused a significant elevation in levels of malonaldehyde and reduced glutathione and sharp inhibition in the activities of super oxide dismutase, catalase, and glutathione-S-transferase; the effect being dose dependent. Carbofuran at different doses also caused sharp reduction in the activity of Na(+)-K(+)-ATPase. The pretreatment of vitamin E, however, showed a significant recovery in these indices. The pretreatment of rats with vitamin E offered protection from carbofuran-induced oxidative stress.
农药会导致氧化应激，并对动物体内的钠钾ATP酶活性产生不利影响。由于呋喃丹对哺乳动物大脑的影响尚未得到充分研究，因此研究了呋喃丹诱导氧化应激和调节钠钾ATP酶活性的能力，以及维生素E对其的改善作用。将大鼠分为六组，给予两种不同剂量的呋喃丹（15%和30%的半数致死剂量），持续15天。结果表明，呋喃丹处理导致丙二醛和还原型谷胱甘肽水平显著升高，超氧化物歧化酶、过氧化氢酶和谷胱甘肽-S-转移酶的活性急剧抑制，且这种效应具有剂量依赖性。不同剂量的呋喃丹还导致钠钾ATP酶活性急剧降低。然而，维生素E预处理使这些指标有显著恢复。用维生素E预处理大鼠可防止呋喃丹诱导的氧化应激。
