Potentiation of nitric oxide formation following bilateral carotid occlusion and focal cerebral ischemia in the rat: in vivo detection of the nitric oxide radical by electron paramagnetic resonance spin trapping.

We have directly demonstrated in vivo that nitric oxide (NO) is produced in the ischemic rat brain. Using diethyldithiocarbamate and Fe as spin-trapping agents, NO spin adducts were detected by cryogenic electron paramagnetic resonance. The cerebral cortex which was exposed to focal ischemia or bilateral carotid artery occlusion generated an increased amount of spin-adducts of NO radicals (g = 2.039, a hyperfine coupling constant aN = 13 gauss). This signal disappeared by the preischemic administration of NG-nitro-L-arginine methylester, a NO synthase inhibitor.