Khramtsov N V, Feshchenko E A, Suslova V A, Shmukler B E, Terpugov B E, Rakitina T V, Atabekova N V, Lipkin V M
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow.
FEBS Lett. 1993 Aug 2;327(3):275-8. doi: 10.1016/0014-5793(93)81003-i.
cDNA clones encoding the beta-subunit of the photoreceptor cGMP phosphodiesterase (PDE) were isolated from a human retina library and their sequence was determined. The encoded polypeptide consists of 854 amino acid residues with a calculated molecular mass of 98,416 Da. Alignment of the deduced amino acid sequence with the earlier analysed alpha-, beta- and alpha'-subunits of bovine and mouse PDEs demonstrates a high homology. Two overlapping recombinant lambda phage clones containing 26 kb of the human PDE beta-subunit gene were isolated from the genomic library. A total nucleotide sequence of exons 4-22 of the PDE beta-subunit gene was established which completely corresponded to the cDNA structure. According to sequence analysis no potential possibility for alternative splicing of the beta-subunit gene was observed between exons 20 and 21 which led to the formation of the beta'-subunit as described for mouse PDE. Polymerase chain reaction (PCR) experiments also confirm the absence of the PDE beta'-subunit in human retina.
从人视网膜文库中分离出编码光感受器环磷酸鸟苷磷酸二酯酶（PDE）β亚基的cDNA克隆，并测定了其序列。编码的多肽由854个氨基酸残基组成，计算分子量为98,416道尔顿。将推导的氨基酸序列与先前分析的牛和小鼠PDE的α、β和α'亚基进行比对，显示出高度同源性。从基因组文库中分离出两个重叠的重组λ噬菌体克隆，它们包含26 kb的人PDEβ亚基基因。确定了PDEβ亚基基因外显子4 - 22的总核苷酸序列，其与cDNA结构完全对应。根据序列分析，未观察到外显子20和21之间β亚基基因发生可变剪接的潜在可能性，而小鼠PDE的可变剪接会导致β'亚基的形成。聚合酶链反应（PCR）实验也证实了人视网膜中不存在PDEβ'亚基。
