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多种16型人乳头瘤病毒糖皮质激素反应元件在转化、瞬时表达及DNA-蛋白质相互作用方面具有功能。

Multiple human papillomavirus type 16 glucocorticoid response elements functional for transformation, transient expression, and DNA-protein interactions.

作者信息

Mittal R, Pater A, Pater M M

机构信息

Division of Basic Medical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, Canada.

出版信息

J Virol. 1993 Sep;67(9):5656-9. doi: 10.1128/JVI.67.9.5656-5659.1993.

DOI:10.1128/JVI.67.9.5656-5659.1993
PMID:8394465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC237971/
Abstract

We have previously shown that human papillomavirus type 16 (HPV-16) can efficiently transform primary baby rat kidney cells in the presence of the steroid hormones progesterone and the glucocorticoid dexamethasone. To study this effect of hormone, different combinations of the previously identified glucocorticoid response element (GRE) at nucleotide 7640 of HPV-16 and the other two GREs that we have recently identified, at nucleotides 7385 and 7474, were mutated. The previously described GRE and the other two GREs were shown to be functional for the induction of transformation by dexamethasone. In addition, transient assays in cervical HeLa cells demonstrated the functional importance of the three individual GREs. Assays for in vitro interaction demonstrated the specific binding of a 97-kDa protein, the glucocorticoid receptor, to both recently identified HPV-16 GREs.

摘要

我们之前已经表明，在类固醇激素孕酮和糖皮质激素地塞米松存在的情况下，16型人乳头瘤病毒（HPV-16）能够有效地转化原代新生大鼠肾细胞。为了研究激素的这种作用，我们对HPV-16核苷酸7640处先前鉴定的糖皮质激素反应元件（GRE）与我们最近鉴定的另外两个位于核苷酸7385和7474处的GRE的不同组合进行了突变。先前描述的GRE以及另外两个GRE被证明对于地塞米松诱导的转化具有功能。此外，在宫颈HeLa细胞中进行的瞬时分析证明了这三个单独的GRE的功能重要性。体外相互作用分析证明了一种97 kDa蛋白质，即糖皮质激素受体，与最近鉴定的两个HPV-16 GRE的特异性结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2fc/237971/363a0b815ace/jvirol00030-0592-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2fc/237971/10d1531ed204/jvirol00030-0592-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2fc/237971/6fa8e6969f6e/jvirol00030-0592-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2fc/237971/363a0b815ace/jvirol00030-0592-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2fc/237971/10d1531ed204/jvirol00030-0592-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2fc/237971/6fa8e6969f6e/jvirol00030-0592-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2fc/237971/363a0b815ace/jvirol00030-0592-c.jpg

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