Matsui F, Oohira A, Shoji R, Kariya Y, Yoshida K
Department of Perinatology, Institute for Developmental Research, Aichi, Japan.
Neurosci Res. 1993 May;16(4):287-92. doi: 10.1016/0168-0102(93)90039-s.
Glycosaminoglycans (GAGs) were isolated from the brains of reeler and normal mice on postnatal days 13 and 20. The GAG content of the reeler mouse brain, based upon the amount of DNA, was about 150% that of the normal mouse brain on both days. The GAGs consisted of chondroitin sulfate (CS), heparan sulfate (HS), hyaluronic acid (HA) and polysialosyl glycopeptides. There was no significant difference in the composition of GAGs isolated from either reeler or normal brain. Repeating disaccharide compositions of CS and HS were also similar in reeler and normal brains. Core proteins of brain chondroitin sulfate proteoglycans (CSPGs), solubilized with phosphate buffered saline, were prepared by digesting purified CSPGs with chondroitinase ABC, and were analyzed by SDS-polyacrylamide slab gel electrophoresis. There was no difference in the composition of core proteins from either reeler or normal brain. These results indicate that, although the GAG content of the reeler mouse brain is higher than the normal, all structural parameters of GAGs/CSPGs so far examined were normal. The rate of synthesis and/or degradation of brain GAGs may be affected in the mutant mouse brain.
在出生后第13天和第20天，从摇晃小鼠和正常小鼠的大脑中分离出糖胺聚糖（GAGs）。基于DNA量计算，摇晃小鼠大脑中的GAG含量在这两天均约为正常小鼠大脑的150%。这些GAGs由硫酸软骨素（CS）、硫酸乙酰肝素（HS）、透明质酸（HA）和多唾液酸糖肽组成。从摇晃小鼠或正常小鼠大脑中分离出的GAGs的组成没有显著差异。在摇晃小鼠和正常小鼠大脑中，CS和HS的重复二糖组成也相似。用磷酸缓冲盐水溶解的脑硫酸软骨素蛋白聚糖（CSPGs）的核心蛋白，通过用硫酸软骨素酶ABC消化纯化的CSPGs来制备，并通过SDS-聚丙烯酰胺平板凝胶电泳进行分析。摇晃小鼠或正常小鼠大脑的核心蛋白组成没有差异。这些结果表明，尽管摇晃小鼠大脑中的GAG含量高于正常小鼠，但到目前为止所检测的GAGs/CSPGs的所有结构参数都是正常的。突变小鼠大脑中脑GAGs的合成和/或降解速率可能受到影响。
