垂体腺苷酸环化酶激活多肽受体的分子克隆与组织分布
Molecular cloning and tissue distribution of a receptor for pituitary adenylate cyclase-activating polypeptide.
作者信息
Hashimoto H, Ishihara T, Shigemoto R, Mori K, Nagata S
机构信息
Osaka Bioscience Institute, Japan.
出版信息
Neuron. 1993 Aug;11(2):333-42. doi: 10.1016/0896-6273(93)90188-w.
Pituitary adenylate cyclase-activating polypeptide (PA-CAP) is a polypeptide hormone related to vasoactive intestinal polypeptide (VIP). Rat PACAP receptor cDNA was isolated from a brain cDNA library by cross-hybridization with rat VIP receptor cDNA. The recombinant PACAP receptor expressed in COS cells bound PACAP with about 1000 times higher affinity than VIP, and PACAP stimulated adenylate cyclase through the cloned PACAP receptor. The rat PACAP receptor consists of 495 amino acids, contains seven transmembrane segments, and has a significant similarity with other Gs-coupled receptors, such as VIP, glucagon, and secretin receptors. PACAP receptor mRNA was abundantly expressed in the brain, but not in the peripheral tissues except for the adrenal gland. In situ hybridization revealed a high level of expression of PACAP receptor mRNA in the hippocampal dentate gyrus, olfactory bulb, and cerebellar cortex.
垂体腺苷酸环化酶激活多肽（PA-CAP）是一种与血管活性肠肽（VIP）相关的多肽激素。通过与大鼠VIP受体cDNA进行交叉杂交，从大鼠脑cDNA文库中分离出大鼠PACAP受体cDNA。在COS细胞中表达的重组PACAP受体与PACAP结合的亲和力比VIP高约1000倍，并且PACAP通过克隆的PACAP受体刺激腺苷酸环化酶。大鼠PACAP受体由495个氨基酸组成，含有七个跨膜区段，并且与其他Gs偶联受体（如VIP、胰高血糖素和促胰液素受体）具有显著的相似性。PACAP受体mRNA在脑中大量表达，但除肾上腺外，在外周组织中不表达。原位杂交显示PACAP受体mRNA在海马齿状回、嗅球和小脑皮质中高水平表达。
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