Gupte M D, Kamat M Y
Food and Fermentation Technology Division, University Department of Chemical Technology, University of Mumbai, Matunga, India.
Folia Microbiol (Praha). 1997;42(6):621-8. doi: 10.1007/BF02815476.
One hundred fifty wild strains of Xanthomonas were isolated on five selective and nonselective media. The comparative effectivity of the five media has been discussed. Fifteen polysaccharide-producing strains shortlisted were identified up to species level. These were studied for polysaccharide production in shake flasks using YM media. The highest final yield of 21.3 g/L of the amount of carbon source supplied has been obtained in the optimum medium in shake-flask experiments from Xanthomonas campestris (ICa-125 strain isolated from cabbage. Rheological properties of the exocellular polysaccharide (EPS) have been compared with standard xanthan. EPS from X. campestris ICa-125 was found to be superior with respect to heat stability and effect of electrolytes as compared to standard xanthan.
在五种选择性和非选择性培养基上分离出150株野油菜黄单胞菌菌株。讨论了这五种培养基的比较有效性。对筛选出的15株产多糖菌株进行了种水平鉴定。使用YM培养基在摇瓶中研究了这些菌株的多糖生产情况。在摇瓶实验中，从野油菜黄单胞菌（从卷心菜中分离出的ICa - 125菌株）的最佳培养基中，以所供应碳源计，获得了最高最终产量21.3 g/L。已将胞外多糖（EPS）的流变学性质与标准黄原胶进行了比较。发现野油菜黄单胞菌ICa - 125的EPS在热稳定性和电解质影响方面优于标准黄原胶。
