Hayashi J
First Department of Surgery, Kurume University School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1993 Jul;94(7):690-701.
DNA and RNA contents in cells of human colorectal cancer transplanted in nude mice were analysed by a flow cytometric method after the cells were stained with Acridine Orange. The results revealed that the method was found to be useful for the analysis of proliferative activity of tumor cells. Relative DNA and RNA contents in the cells were expressed as DNA index (DI) and RNA index (RI), respectively. Of the six colorectal cancer materials used to measure DI, RI and their tumor doubling time (TD), KLM3, KLM1, KRK1 and KRK4 are well differentiated adenocarcinoma, COLO 205 is poorly differentiated adenocarcinoma, and KSK1 is well differentiated mucinous carcinoma. A significant correlation was found between RI and TD in the total six models (n = 92, r = 0.49, p < 0.001) and every models of KLM3 (n = 27, r = -0.67, p < 0.001) and KRK4 (n = 10, r = -0.65, p < 0.05), while no correlation between DI and TD was found in all models. The results indicated that the measurement of the cellular RNA content is a useful index to evaluate the proliferative activity of human solid tumors.
用吖啶橙对裸鼠移植的人结肠癌细胞进行染色后，采用流式细胞术分析细胞中的DNA和RNA含量。结果表明，该方法可用于分析肿瘤细胞的增殖活性。细胞中的相对DNA和RNA含量分别表示为DNA指数（DI）和RNA指数（RI）。在用于测量DI、RI及其肿瘤倍增时间（TD）的六种结肠直肠癌材料中，KLM3、KLM1、KRK1和KRK4为高分化腺癌，COLO 205为低分化腺癌，KSK1为高分化黏液癌。在全部六个模型（n = 92，r = 0.49，p < 0.001）以及KLM3的每个模型（n = 27，r = -0.67，p < 0.001）和KRK4的每个模型（n = 10，r = -0.65，p < 0.05）中，发现RI与TD之间存在显著相关性，而在所有模型中均未发现DI与TD之间存在相关性。结果表明，细胞RNA含量的测定是评估人实体瘤增殖活性的有用指标。
