Muravyeva O V, Cragoe E J, Margolis L B
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Biochim Biophys Acta. 1993 Sep 13;1178(3):267-72. doi: 10.1016/0167-4889(93)90203-2.
The pH dependence of spreading of normal mouse embryo fibroblasts was investigated. It was shown that, in contrast to DNA synthesis, cell spreading did not depend on intracellular pH in the same pH range. Serum growth factors had no influence on intracellular pH in the suspended cells. Spreading of the fibroblasts on poly-L-lysine-coated coverslips in a serum-free medium did not alter intracellular pH. Cytoplasm alkalynization accompanying the fibroblasts spreading in a medium with serum is suggested to be due to the effect of serum growth factors on the spread cells. The inhibitor of the Na+ H(+)-antiporter, MIBA, and the inhibitor of PKC, staurosporin, as well as the PMA-induced cell depletion of PKC prevented pH increase, but had no effect on the spreading itself. It is concluded that the pH increase observed during fibroblasts spreading in a serum-containing medium is not required for the spreading itself being due to the activation of both the Na+/H(+)-antiporter and PKC.
研究了正常小鼠胚胎成纤维细胞铺展的pH依赖性。结果表明，与DNA合成不同，在相同的pH范围内，细胞铺展不依赖于细胞内pH。血清生长因子对悬浮细胞的细胞内pH没有影响。在无血清培养基中，成纤维细胞在聚-L-赖氨酸包被的盖玻片上的铺展不会改变细胞内pH。成纤维细胞在含血清培养基中铺展时伴随的细胞质碱化被认为是由于血清生长因子对铺展细胞的作用。Na + H(+)反向转运体抑制剂MIBA、蛋白激酶C（PKC）抑制剂星形孢菌素以及PMA诱导的PKC细胞耗竭可阻止pH升高，但对铺展本身没有影响。得出的结论是，在含血清培养基中，成纤维细胞铺展过程中观察到的pH升高对于铺展本身并非必需，这是由于Na + /H(+)反向转运体和PKC的激活所致。
