Kazuno Y, Homma T, Nemoto Y, Matsuoka H
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Biochim Biophys Acta. 1993 Sep 13;1178(3):299-301. doi: 10.1016/0167-4889(93)90207-6.
A flow system has been developed for the continual microscopic observation of cultured nerve cells. The undefined effects of coexisting cells could be efficiently reduced by the continuous flow of a medium. The present flow system was useful also for the experiments requiring medium exchanges. Its performance was demonstrated by the repeated actions of NGF or db-cAMP to PC12h-R cells. The neurite projection and its retraction were repeatedly observed by the supply and the removal of those factors.
已开发出一种流动系统，用于对培养的神经细胞进行连续显微镜观察。通过培养基的连续流动，可以有效减少共存细胞的不确定影响。本流动系统对于需要更换培养基的实验也很有用。通过对PC12h-R细胞反复施加神经生长因子（NGF）或二丁酰环磷腺苷（db-cAMP），证明了该系统的性能。通过供应和去除这些因子，反复观察到神经突的伸出和回缩。
