Tomura A, Ishikawa T, Sagara Y, Miki T, Sekimizu K
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
FEBS Lett. 1993 Aug 30;329(3):287-90. doi: 10.1016/0014-5793(93)80239-q.
We report that phosphatidylglycerol is required for flagellation of Escherichia coli. Cells carrying the pgsA3 mutation did not form swarm rings in semisolid agar. P1 transduction experiments revealed that the potential for phosphatidylglycerol synthesis and for the formation of swarm rings was co-transducible. The pgsA3 mutant transformed with the wild type pgsA+ gene cloned into the R-plasmid vector had the potential for both phosphatidylglycerol synthesis and cell motility. Electromicroscopic and SDS-PAGE analyses showed that the pgsA3 mutation causes the lack of flagellation.
我们报告称，磷脂酰甘油是大肠杆菌鞭毛形成所必需的。携带pgsA3突变的细胞在半固体琼脂中不会形成群体环。P1转导实验表明，磷脂酰甘油合成能力和群体环形成能力是共转导的。用克隆到R质粒载体中的野生型pgsA+基因转化的pgsA3突变体具有磷脂酰甘油合成能力和细胞运动能力。电子显微镜和SDS-PAGE分析表明，pgsA3突变导致鞭毛缺失。
