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大肠杆菌dnaA突变体中鞭毛形成的丧失。

Loss of flagellation in dnaA mutants of Escherichia coli.

作者信息

Mizushima T, Tomura A, Shinpuku T, Miki T, Sekimizu K

机构信息

Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.

出版信息

J Bacteriol. 1994 Sep;176(17):5544-6. doi: 10.1128/jb.176.17.5544-5546.1994.

DOI:10.1128/jb.176.17.5544-5546.1994
PMID:8071236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196746/
Abstract

A dnaA46 mutant of Escherichia coli showed loss of motility at 37 degrees C, a permissive temperature for cell growth of this mutant. Other dnaA mutations near the middle of the gene also caused an immotile phenotype. The amount of flagellin was much less in the dnaA46 mutant than in the wild-type control, as was the promoter activity. DnaA protein may play an important role in expression of the fliC gene.

摘要

大肠杆菌的dnaA46突变体在37℃（该突变体细胞生长的允许温度）下表现出运动性丧失。基因中部附近的其他dnaA突变也导致了不运动的表型。与野生型对照相比，dnaA46突变体中鞭毛蛋白的量少得多，启动子活性也是如此。DnaA蛋白可能在fliC基因的表达中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bf/196746/5dd9b1204413/jbacter00035-0362-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bf/196746/3ea575f22f3f/jbacter00035-0361-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bf/196746/5dd9b1204413/jbacter00035-0362-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bf/196746/3ea575f22f3f/jbacter00035-0361-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44bf/196746/5dd9b1204413/jbacter00035-0362-a.jpg

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本文引用的文献

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Purified dnaA protein in initiation of replication at the Escherichia coli chromosomal origin of replication.纯化的DnaA蛋白在大肠杆菌染色体复制起点处启动复制过程中的作用。
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