Ohba A, Inoue R, Akimitsu N, Mizushima T, Sekimizu K
Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Biol Pharm Bull. 1998 Nov;21(11):1139-41. doi: 10.1248/bpb.21.1139.
We identified proteins whose amounts were altered in an Escherichia coli pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. Proteins whose amounts were increased in the mutant were protease Do, periplasmic oligopeptide-binding protein, tryptophanase, and an unidentified protein, while the decreased one was flagellin. Transformation of the mutant with a plasmid containing the wild type pgsA gene complemented the phenotype, indicating that the pgsA3 mutation is responsible for the phenotype.
我们鉴定了在缺乏合成磷脂酰甘油磷酸（磷脂酰甘油的前体）能力的大肠杆菌pgsA3突变体中含量发生改变的蛋白质。在该突变体中含量增加的蛋白质有蛋白酶Do、周质寡肽结合蛋白、色氨酸酶和一种未鉴定的蛋白质，而含量减少的是鞭毛蛋白。用含有野生型pgsA基因的质粒转化该突变体可互补该表型，表明pgsA3突变是造成该表型的原因。
