Donelli G, Superti F, Tinari A, Marziano M L, Caione D, Concato C, Menichella D
Laboratorio di Ultrastrutture, Istituto Superiore di Sanità, Rome, Italy.
New Microbiol. 1993 Jul;16(3):215-25.
During the period May 1987-January 1989, faecal samples from 417 paediatric inpatients admitted to the main paediatric hospital in Rome were screened by direct electron microscopy and rotavirus enzyme-linked immunosorbent assay. Rotaviruses were detected in 18.2% of cases and adenoviruses in 7%, whereas astroviruses were found in 1% of cases. Different percentages of rotavirus excretors were revealed by enzyme-linked immunosorbent assay and electron microscopy. This discrepancy seems to be due to false positive results introduced by enzyme-linked immunosorbent assay. Analysis of electron microscopy-positive samples by rotaviral RNA polyacrylamide gel electrophoresis showed different electropherotypes of rotavirus among which a single, largely predominant long electropherotype (55.4%) was revealed. Short electropherotype subgroup I rotaviruses were demonstrated in about 10.7% of samples.
在1987年5月至1989年1月期间，对罗马主要儿童医院收治的417名儿科住院患者的粪便样本进行了直接电子显微镜检查和轮状病毒酶联免疫吸附测定筛查。轮状病毒在18.2%的病例中被检测到，腺病毒在7%的病例中被检测到，而星状病毒在1%的病例中被发现。酶联免疫吸附测定和电子显微镜检查显示出不同比例的轮状病毒排泄者。这种差异似乎是由于酶联免疫吸附测定引入的假阳性结果所致。通过轮状病毒RNA聚丙烯酰胺凝胶电泳对电子显微镜检查呈阳性的样本进行分析，结果显示轮状病毒存在不同的电泳型，其中一种单一的、占主导地位的长电泳型（55.4%）被发现。短电泳型I亚组轮状病毒在约10.7%的样本中被证实。
