微量滴定板酶免疫测定法检测血液中念珠菌属经聚合酶链反应扩增的DNA。
Microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species in blood.
作者信息
Fujita S, Lasker B A, Lott T J, Reiss E, Morrison C J
机构信息
Central Clinical Laboratory, Kanazawa University Hospital, Kanazawa, Japan.
出版信息
J Clin Microbiol. 1995 Apr;33(4):962-7. doi: 10.1128/jcm.33.4.962-967.1995.
Abstract
We developed a microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species. Nucleotide sequences derived from the internal transcribed spacer (ITS) region of fungal rDNA were used to develop species-specific oligonucleotide probes for Candida albicans, C. tropicalis, C. parapsilosis, and C. krusei. No cross-hybridization was detected with any other fungal, bacterial, or human DNAs tested. In contrast, a C. (Torulopsis) glabrata probe cross-reacted with Saccharomyces cerevisiae DNA but with no other DNAs tested. Genomic DNA purified from C. albicans blastoconidia suspended in blood was amplified by PCR with fungus-specific universal primers ITS3 and ITS4. With the C. albicans-specific probe labeled with digoxigenin, a biotinylated capture probe, and streptavidin-coated microtitration plates, amplified DNA from a few as two C. albicans cells per 0.2 ml of blood could be detected by enzyme immunoassay.
摘要
我们开发了一种微量滴定板酶免疫测定法，用于检测念珠菌属经聚合酶链反应（PCR）扩增的DNA。从真菌核糖体DNA的内部转录间隔区（ITS）区域获得的核苷酸序列，被用于开发针对白色念珠菌、热带念珠菌、近平滑念珠菌和克柔念珠菌的种特异性寡核苷酸探针。在所检测的任何其他真菌、细菌或人类DNA中均未检测到交叉杂交现象。相比之下，光滑念珠菌（Torulopsis）探针与酿酒酵母DNA发生交叉反应，但与所检测的其他DNA均无交叉反应。用真菌特异性通用引物ITS3和ITS4通过PCR扩增从悬浮于血液中的白色念珠菌芽生孢子中纯化的基因组DNA。使用用地高辛标记的白色念珠菌特异性探针、生物素化捕获探针和链霉抗生物素蛋白包被的微量滴定板，通过酶免疫测定法能够检测出每0.2 ml血液中低至两个白色念珠菌细胞的扩增DNA。
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本文引用的文献
1
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Nucleic Acids Res. 1993 Aug 11;21(16):3905-6. doi: 10.1093/nar/21.16.3905.
2
Application of the polymerase chain reaction to the diagnosis of candidosis by amplification of an HSP 90 gene fragment.通过扩增热休克蛋白90（HSP 90）基因片段，将聚合酶链反应应用于念珠菌病的诊断。
J Med Microbiol. 1993 Sep;39(3):233-8. doi: 10.1099/00222615-39-3-233.
3
Polymerase chain reaction for the diagnosis of candidemia.用于诊断念珠菌血症的聚合酶链反应
J Infect Dis. 1993 Sep;168(3):779-83. doi: 10.1093/infdis/168.3.779.
4
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J Clin Microbiol. 1993 Dec;31(12):3344-7. doi: 10.1128/jcm.31.12.3344-3347.1993.
5
Nonculture methods for diagnosis of disseminated candidiasis.用于诊断播散性念珠菌病的非培养方法。
Clin Microbiol Rev. 1993 Oct;6(4):311-23. doi: 10.1128/CMR.6.4.311.
6
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7
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Yeast. 1993 Nov;9(11):1199-206. doi: 10.1002/yea.320091106.
8
Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis.利用聚合酶链反应（PCR）扩增和限制性内切酶分析检测及鉴别临床标本中的真菌。
J Med Vet Mycol. 1993;31(1):65-75. doi: 10.1080/02681219380000071.
9
Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment.通过对细胞色素P-450羊毛甾醇-α-脱甲基酶（L1A1）基因片段进行种特异性巢式PCR扩增，快速检测和鉴定临床标本中的白色念珠菌和光滑念珠菌（念珠菌属）。
J Clin Microbiol. 1994 Aug;32(8):1902-7. doi: 10.1128/jcm.32.8.1902-1907.1994.
10
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J Infect Dis. 1982 Jan;145(1):45-56. doi: 10.1093/infdis/145.1.45.
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