Egyed L, Ballagi-Pordány A, Bartha A, Belák S
Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest, Hungary.
J Clin Microbiol. 1996 May;34(5):1091-5. doi: 10.1128/jcm.34.5.1091-1095.1996.
The in vivo distribution of bovine herpesvirus type 4 (BHV-4) was examined by testing nasal and conjunctival exudates, peripheral blood leukocytes, and various organs of experimentally infected calves. For virus detection, a nested PCR assay, virus isolation, and immunohistochemistry were applied. The nervous system and the muscles were free of viral DNA. Liver and intestinal lymph nodes contained low amounts of virus (less than two copies per 1 microgram of cellular DNA). Intestinal, tonsil, thymus, and kidney tissues contained more viral DNA copies (5 to 50 copies per 1 microgram of cellular DNA). The highest amounts of BHV-4 DNA (50 to 500 copies per 1 microgram of cellular DNA) were found in the spleen, lungs, trachea, and nasal epithelium. Amplification of DNA from blood lymphocytes through postinoculation (p.i.) day 48 proved that the virus started to replicate in these cells immediately after inoculation of the calves and that intensive virus growth took place during the 7 to 8 weeks of the infection. The number of virus-infected lymphocytes reached the maximum on p.i. days 22 to 26 and slowly declined thereafter. Virus-infected cells were found only in the spleen on p.i. day 48 by immunohistochemistry. Western blotting (immunoblotting) detected signs of an immune response against 9 of the 29 BHV-4 proteins.
通过检测实验感染犊牛的鼻和结膜渗出物、外周血白细胞及各种器官，研究了牛4型疱疹病毒（BHV - 4）的体内分布。为进行病毒检测，应用了巢式PCR检测、病毒分离和免疫组织化学方法。神经系统和肌肉未检测到病毒DNA。肝脏和肠淋巴结含有少量病毒（每1微克细胞DNA中少于两个拷贝）。肠、扁桃体、胸腺和肾组织含有较多病毒DNA拷贝（每1微克细胞DNA中5至50个拷贝）。在脾脏、肺、气管和鼻上皮中发现了最高量的BHV - 4 DNA（每1微克细胞DNA中50至500个拷贝）。接种后48天从血液淋巴细胞中扩增DNA证明，犊牛接种病毒后病毒立即开始在这些细胞中复制，并且在感染的7至8周内病毒大量增殖。病毒感染淋巴细胞的数量在接种后22至26天达到最大值，此后缓慢下降。接种后48天通过免疫组织化学仅在脾脏中发现了病毒感染细胞。蛋白质印迹法（免疫印迹法）检测到针对29种BHV - 4蛋白中的9种的免疫反应迹象。
