通过聚合酶链反应快速诊断禽传染性支气管炎病毒
Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction.
作者信息
Falcone E, D'Amore E, Di Trani L, Sili A, Tollis M
机构信息
Istituto Superiore di Sanità, Laboratorio di Medicina Veterinaria, Rome, Italy.
出版信息
J Virol Methods. 1997 Mar;64(2):125-30. doi: 10.1016/s0166-0934(96)02151-9.
Abstract
A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tissues of naturally infected birds. Viral RNA was then amplified and identified by a nested RT-PCR assay using two sets of primers flanking a well-conserved region of the nucleocapsid gene. The selected IBV nucleocapsid sequence was detected successfully by simple direct electrophoresis of amplified material.
摘要
为了直接在组织样本中检测传染性支气管炎病毒（IBV），开发了一种简单、灵敏且特异的聚合酶链反应（PCR）方法。从用不同IBV毒株进行实验性感染的尿囊液和细胞培养物以及自然感染禽类的组织中提取病毒RNA。然后使用两组位于核衣壳基因保守区域两侧的引物，通过巢式逆转录PCR检测法对病毒RNA进行扩增和鉴定。通过对扩增产物进行简单的直接电泳，成功检测到了所选的IBV核衣壳序列。
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本文引用的文献
1
Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology.聚合酶链反应（PCR）在兽医诊断病毒学中的应用。
Vet Res Commun. 1993;17(1):55-72. doi: 10.1007/BF01839180.
2
Polymerase chain reaction and a biotin-labeled DNA probe for detection of infectious bronchitis virus in chickens.用于检测鸡传染性支气管炎病毒的聚合酶链反应及生物素标记的DNA探针
Avian Dis. 1993 Jan-Mar;37(1):149-56.
3
A 'novel' infectious bronchitis strain infecting broiler chickens in Italy.一种在意大利感染肉鸡的“新型”传染性支气管炎毒株。
Zentralbl Veterinarmed B. 1994 Apr;41(2):83-9. doi: 10.1111/j.1439-0450.1994.tb00211.x.
4
A novel variant of avian infectious bronchitis virus resulting from recombination among three different strains.一种由三种不同毒株重组产生的新型禽传染性支气管炎病毒变种。
Arch Virol. 1995;140(2):259-71. doi: 10.1007/BF01309861.
5
Avian infectious bronchitis.禽传染性支气管炎
Adv Vet Sci Comp Med. 1970;14:105-48.
6
Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.通过聚合酶催化的链式反应在体外特异性合成DNA。
Methods Enzymol. 1987;155:335-50. doi: 10.1016/0076-6879(87)55023-6.
7
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.用于镰状细胞贫血诊断的β-珠蛋白基因组序列的酶促扩增及限制性酶切位点分析。
Science. 1985 Dec 20;230(4732):1350-4. doi: 10.1126/science.2999980.
8
Sequences of the nucleocapsid genes from two strains of avian infectious bronchitis virus.两种禽传染性支气管炎病毒毒株核衣壳基因的序列
J Gen Virol. 1985 Mar;66 ( Pt 3):573-80. doi: 10.1099/0022-1317-66-3-573.
9
Cloning and sequencing of genes encoding structural proteins of avian infectious bronchitis virus.
Virology. 1988 Aug;165(2):589-95. doi: 10.1016/0042-6822(88)90603-4.
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