通过聚合酶链反应快速诊断禽传染性支气管炎病毒
Rapid diagnosis of avian infectious bronchitis virus by the polymerase chain reaction.
作者信息
Falcone E, D'Amore E, Di Trani L, Sili A, Tollis M
机构信息
Istituto Superiore di Sanità, Laboratorio di Medicina Veterinaria, Rome, Italy.
出版信息
J Virol Methods. 1997 Mar;64(2):125-30. doi: 10.1016/s0166-0934(96)02151-9.
Abstract
A simple, sensitive and specific polymerase chain reaction (PCR) procedure was developed in order to detect infectious bronchitis virus (IBV) directly in tissue samples. Viral RNA was extracted from allantoic fluids and cell cultures infected experimentally with different strains of IBV and from tissues of naturally infected birds. Viral RNA was then amplified and identified by a nested RT-PCR assay using two sets of primers flanking a well-conserved region of the nucleocapsid gene. The selected IBV nucleocapsid sequence was detected successfully by simple direct electrophoresis of amplified material.
摘要
为了直接在组织样本中检测传染性支气管炎病毒（IBV），开发了一种简单、灵敏且特异的聚合酶链反应（PCR）方法。从用不同IBV毒株进行实验性感染的尿囊液和细胞培养物以及自然感染禽类的组织中提取病毒RNA。然后使用两组位于核衣壳基因保守区域两侧的引物，通过巢式逆转录PCR检测法对病毒RNA进行扩增和鉴定。通过对扩增产物进行简单的直接电泳，成功检测到了所选的IBV核衣壳序列。
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