Carranza M C, Simón M A, Torres A, Romero B, Calle C
Department of Biochemistry, School of Medicine, Complutense University, Madrid, Spain.
J Endocrinol Invest. 1993 Jun;16(6):439-42. doi: 10.1007/BF03348878.
The presence of glucagon receptors on human adipocytes has not yet been described. In this work we present an exceptional case of glucagon binding to human adipocytes taken from a malignant tumor of adipose tissue of a patient with a liposarcoma. Binding analysis revealed that the total number of glucagon receptors on liposarcoma-cells was 99,000 and the apparent receptor affinity (ED:50) was 5 x 10(-9) M. Despite the presence of these specific receptors, glucagon was unable to induce a lipolytic response, or to activate the adenylate-cyclase system in these liposarcoma-cells. Whether the induction of glucagon receptors is a specific process of the tumor biology remains to be elucidated.
人类脂肪细胞上胰高血糖素受体的存在尚未见报道。在本研究中，我们展示了一个特殊病例，即从一名脂肪肉瘤患者的脂肪组织恶性肿瘤中获取的人类脂肪细胞与胰高血糖素结合。结合分析显示，脂肪肉瘤细胞上胰高血糖素受体的总数为99,000，表观受体亲和力（ED50）为5×10⁻⁹ M。尽管存在这些特异性受体，但胰高血糖素无法在这些脂肪肉瘤细胞中诱导脂解反应或激活腺苷酸环化酶系统。胰高血糖素受体的诱导是否是肿瘤生物学的一个特定过程仍有待阐明。
