Swenson S L, McMillen J, Hill H T
Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames 50011.
J Vet Diagn Invest. 1993 Jul;5(3):347-50. doi: 10.1177/104063879300500307.
The differential pseudorabies virus (PRV) vaccines currently in use in the USA have deletions of the genes coding for the glycoprotein I (gI) and/or glycoprotein X (gpX). The absence of gI and/or gpX allow for the serologic differentiation of vaccinated swine from PRV-infected swine using differential enzyme-linked immunosorbent assays (ELISAs). A newly developed pseudorabies vaccine virus has 4 deletions of the viral genome: the genes coding for gI, gpX, and thymidine kinase and a portion of the repeat region to attenuate the virus. The purpose of this work was to evaluate the diagnostic compatibility of the gI/gpX gene-deleted vaccine with 3 differential vaccines and 3 differential ELISAs currently in use. Pigs vaccinated 3 times with the gI/gpX gene-deleted vaccine remained seronegative on the 3 differential ELISAs tested. Pigs previously vaccinated with either a gI or gpX gene-deleted vaccine and then vaccinated with the gI/gpX gene-deleted vaccine remained seronegative on the respective gI or gpX differential assay.
美国目前使用的鉴别性伪狂犬病病毒（PRV）疫苗缺失编码糖蛋白I（gI）和/或糖蛋白X（gpX）的基因。gI和/或gpX的缺失使得能够使用鉴别性酶联免疫吸附测定（ELISA）对接种疫苗的猪和感染PRV的猪进行血清学区分。一种新开发的伪狂犬病疫苗病毒有4个病毒基因组缺失：编码gI、gpX和胸苷激酶的基因以及部分重复区域，以减弱病毒毒性。这项工作的目的是评估gI/gpX基因缺失疫苗与目前使用的3种鉴别性疫苗和3种鉴别性ELISA的诊断兼容性。用gI/gpX基因缺失疫苗接种3次的猪在测试的3种鉴别性ELISA上仍呈血清阴性。先前接种过gI或gpX基因缺失疫苗，然后接种gI/gpX基因缺失疫苗的猪在各自的gI或gpX鉴别试验中仍呈血清阴性。
