玉米元件Ds在拟南芥中的转座模式。
Transposition pattern of the maize element Ds in Arabidopsis thaliana.
作者信息
Bancroft I, Dean C
机构信息
AFRC Institute of Plant Science Research, Cambridge Laboratory, John Innes Centre, Colney, Norwich, England.
出版信息
Genetics. 1993 Aug;134(4):1221-9. doi: 10.1093/genetics/134.4.1221.
Abstract
As part of establishing an efficient transposon tagging system in Arabidopsis using the maize elements Ac and Ds, we have analyzed the inheritance and pattern of Ds transposition in four independent Arabidopsis transformants. A low proportion (33%) of plants inheriting the marker used to monitor excision contained a transposed Ds. Selection for the transposed Ds increased this to at least 49%. Overall, 68% of Ds transpositions inherited with the excision marker were to genetically linked sites; however, the distribution of transposed elements varied around the different donor sites. Mapping of transposed Ds elements that were genetically unlinked to the donor site showed that a proportion (3 of 11 tested) integrated into sites which were still physically linked.
摘要
作为利用玉米元件Ac和Ds在拟南芥中建立高效转座子标签系统的一部分，我们分析了四个独立的拟南芥转化体中Ds转座的遗传情况和模式。继承用于监测切除的标记的植物中，只有一小部分（33%）含有转座的Ds。对转座的Ds进行选择后，这一比例至少提高到了49%。总体而言，与切除标记一起遗传的Ds转座中有68%发生在基因连锁位点；然而，转座元件在不同供体位点周围的分布有所不同。对与供体位点没有基因连锁的转座Ds元件进行定位发现，有一部分（11个测试中有3个）整合到了仍然存在物理连锁的位点。
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