Garg T K, Mittal A K
Department of Zoology, Banaras Hindu University, Varanasi, India.
Biomed Environ Sci. 1993 Jun;6(2):119-33.
Response of mucous cells, in the epidermis of the catfish Clarias batrachus, to a sublethal concentration of sodium dodecyl sulfate was studied and correlated with the alteration in the status of their secretory activity after different durations of the treatment. At 4h, 8h, 24h, 48h and 72h of the detergent treatment, most mucous cells attained voluminous dimensions and appeared closely approximating to or even overlapping the adjacent ones. At 12h, 36h, and 60h, in contrast, the mucous cells appeared small and slender, and seemed to be located at greater intervals. These changes in dimensions were responsible for the apparent increase or decrease in the density of mucous cells after different durations of detergent treatment. Statistically, however, no significant change was observed in the total number of the mucous cells throughout the experiment. The mucous cells appeared enlarged towards the end of the experiment signifying increased mucus production. This is considered as an adaptation, for protection, assisting the fish to adjust to the changed environment. A shift in the histochemical nature of the secretory contents at the middle and basal parts of the mucous cells, from a mixture of neutral and acid glycoproteins to neutral glycoproteins, during the early stages of the treatment suggests that acid moities could not simultaneously be synthesized as an immediate response to enhanced mucous secretion. The apical parts of the mucous cells, however, showed no histochemical change throughout the experiment.
研究了鲶鱼（胡子鲶）表皮黏液细胞对亚致死浓度十二烷基硫酸钠的反应，并将其与处理不同时长后分泌活动状态的变化相关联。在洗涤剂处理的4小时、8小时、24小时、48小时和72小时时，大多数黏液细胞体积增大，看起来与相邻细胞紧密相邻甚至重叠。相反，在12小时、36小时和60小时时，黏液细胞显得小而细长，且似乎间隔更大。这些尺寸变化导致洗涤剂处理不同时长后黏液细胞密度明显增加或减少。然而，从统计学上看，在整个实验过程中黏液细胞总数未观察到显著变化。在实验末期黏液细胞出现增大，表明黏液分泌增加。这被认为是一种适应性变化，用于保护，帮助鱼适应变化的环境。在处理早期，黏液细胞中部和基部分泌内容物的组织化学性质从酸性和中性糖蛋白混合物转变为中性糖蛋白，这表明酸性部分不能作为对黏液分泌增强的即时反应同时合成。然而，黏液细胞顶端在整个实验过程中未显示组织化学变化。
