Perez R P, Godwin A K, Handel L M, Hamilton T C
Department of Medicine, Roswell Park Cancer Institute, Buffalo, New York 14263.
Eur J Cancer. 1993;29A(3):395-9. doi: 10.1016/0959-8049(93)90394-u.
An assay based upon quantitative staining of cellular protein by sulforhodamine B (SRB) has recently been adopted by the NCI for large-scale screening of new drugs. However, there are few data available regarding whether the SRB assay is comparable to other established methods. Cisplatin cytotoxicity was determined in 16 human ovarian carcinoma cell lines by both SRB and clonogenic assays, and by microtetrazolium (MTT) assay in seven cell lines. Cell lines were derived from untreated patients (some of which were selected for cisplatin resistance in vitro) and from patients clinically refractory to cisplatin-based chemotherapy. There was excellent linear correlation between SRB staining and cell number in all cell lines (r = 0.972-0.999). IC50 values obtained by the SRB and clonogenic assay (r = 0.824, P = 0.000022) were highly correlated, although values obtained in the SRB assay were uniformly higher. IC50 values obtained by SRB assay also correlated well with results obtained by MTT assay (r = 0.906, P = 0.0010). Overall, the SRB assay permitted rapid and reliable assessment of cisplatin sensitivity in these cell lines and compared favourably with clonogenic and MTT assays.
美国国立癌症研究所（NCI）最近采用了一种基于用磺酰罗丹明B（SRB）对细胞蛋白进行定量染色的检测方法来大规模筛选新药。然而，关于SRB检测方法是否与其他既定方法具有可比性的可用数据很少。通过SRB检测和克隆形成检测，以及在7种细胞系中通过微量四氮唑蓝（MTT）检测，测定了16种人卵巢癌细胞系中的顺铂细胞毒性。细胞系来源于未经治疗的患者（其中一些在体外被选择用于顺铂耐药）以及临床上对基于顺铂的化疗难治的患者。在所有细胞系中，SRB染色与细胞数量之间存在极好的线性相关性（r = 0.972 - 0.999）。通过SRB检测和克隆形成检测获得的IC50值高度相关（r = 0.824，P = 0.000022），尽管在SRB检测中获得的值普遍较高。通过SRB检测获得的IC50值也与通过MTT检测获得的结果良好相关（r = 0.906，P = 0.0010）。总体而言，SRB检测能够快速、可靠地评估这些细胞系中的顺铂敏感性，并且与克隆形成检测和MTT检测相比具有优势。
