Keepers Y P, Pizao P E, Peters G J, van Ark-Otte J, Winograd B, Pinedo H M
Department of Oncology, Free University Hospital, Amsterdam, The Netherlands.
Eur J Cancer. 1991;27(7):897-900. doi: 10.1016/0277-5379(91)90142-z.
The sulforhodamine B (SRB) protein stain assay was compared with the tetrazolium (MTT) colorimetric assay for in vitro chemosensitivity testing of various human tumour cell lines. The SRB assay provided a better linearity with cell number and a higher sensitivity, and its staining was not cell-line dependent. In contrast to the MTT assay, the SRB assay stained recently lysed cells. Cell debris, however, was not stained by SRB and therefore the drug sensitivity data were not affected.
将磺酰罗丹明B（SRB）蛋白质染色测定法与四氮唑盐（MTT）比色测定法进行比较，用于各种人类肿瘤细胞系的体外化学敏感性测试。SRB测定法与细胞数量具有更好的线性关系和更高的灵敏度，并且其染色不依赖于细胞系。与MTT测定法不同，SRB测定法可对最近裂解的细胞进行染色。然而，细胞碎片不会被SRB染色，因此药物敏感性数据不受影响。
