Heldin N E, Usuki K, Bergh J, Westermark B, Heldin C H
Department of Pathology, University Hospital, Uppsala, Sweden.
Br J Cancer. 1993 Oct;68(4):708-11. doi: 10.1038/bjc.1993.414.
In the present investigation we have studied the expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) in ten different human lung carcinoma cell lines, four small cell carcinomas and six non-small cell carcinomas. None of the small-cell lung carcinoma cell lines demonstrated expression of PD-ECGF/TP mRNA. However, four of six of the non-small cell carcinoma cell lines expressed the 1.8 kb PD-ECGF/TP transcript. The cell lines derived from the single squamous cell carcinoma and the two adenocarcinomas expressed the PD-ECGF/TP mRNA, and were found to have the corresponding protein both in cell lysates and conditioned media as determined both by immunoblotting and measurement of thymidine phosphorylase activity. Only one of three studied large cell carcinoma cell lines expressed low levels of PD-ECGF/TP mRNA, but the corresponding PD-ECGF/TP protein was not demonstrated by immunoblotting.
在本研究中，我们研究了血小板衍生内皮细胞生长因子/胸苷磷酸化酶（PD-ECGF/TP）在十种不同的人肺癌细胞系中的表达情况，其中包括四种小细胞癌和六种非小细胞癌。所有小细胞肺癌细胞系均未显示PD-ECGF/TP mRNA的表达。然而，六种非小细胞癌细胞系中有四种表达了1.8 kb的PD-ECGF/TP转录本。来自单一鳞状细胞癌和两种腺癌的细胞系表达了PD-ECGF/TP mRNA，并且通过免疫印迹和胸苷磷酸化酶活性测定发现，在细胞裂解物和条件培养基中均存在相应的蛋白质。在所研究的三种大细胞癌细胞系中，只有一种表达了低水平的PD-ECGF/TP mRNA，但免疫印迹未显示相应的PD-ECGF/TP蛋白。
