Söderdahl G, Betsholtz C, Johansson A, Nilsson K, Bergh J
Department of Pathology, University of Uppsala, Akademiska sjukhuset, Sweden.
Int J Cancer. 1988 Apr 15;41(4):636-41. doi: 10.1002/ijc.2910410426.
We have investigated a panel of human lung cancer cell lines representing the major groups of lung cancer, i.e., small-cell carcinoma (SCC) and the group of non-SCC, consisting of squamous-cell carcinoma (SQC), adenocarcinoma (ADC) and large-cell carcinoma (LCC), for their expression of certain growth factor genes. Messenger RNA from each cell line was hybridized with probes for platelet-derived growth factor (PDGF) A- and B-chains, insulin-like growth factor (IGF)-I and -II, transforming growth factor (TGF)-alpha and -beta, epidermal growth factor (EGF) as well as a probe for the EGF receptor. All non-SCC cell lines examined showed expression of the PDGF A-chain gene. The PDGF beta-chain and TGF-beta genes were expressed in all non-SCC cell lines but one, H-125 (ADC). TGF-alpha gene expression was demonstrated in the SQC cell line U-1752, in both ADC cell lines (H-23 and H-125) and in one of the 3 LCC cell lines, U-1810. IGF-II was only transcribed in the LCC cell line U-1810. The EGF-receptor was detected in all non-SCC cell lines but one, H-661 (LCC). Neither IGF-I nor EGF transcripts could be seen in any of the 10 cell lines examined. In contrast to the non-SCC cell lines, the 4 SCC lines were constantly negative for the probes employed in this study. The frequent and heterogeneous expression of growth factor transcripts in all non-SCC studied, but not SCC-cell lines, may contribute to the difference in biological behaviour observed in vivo and in vitro between the 2 major lung cancer entities.
我们研究了一组代表肺癌主要类型的人肺癌细胞系，即小细胞癌（SCC）以及由鳞状细胞癌（SQC）、腺癌（ADC）和大细胞癌（LCC）组成的非小细胞癌组，检测了它们某些生长因子基因的表达情况。从每个细胞系提取的信使核糖核酸（mRNA）与血小板衍生生长因子（PDGF）A链和B链、胰岛素样生长因子（IGF）-I和-II、转化生长因子（TGF）-α和-β、表皮生长因子（EGF）的探针以及EGF受体的探针进行杂交。所有检测的非小细胞癌细胞系均显示PDGF A链基因的表达。除一个细胞系H-125（ADC）外，所有非小细胞癌细胞系均表达PDGF β链和TGF-β基因。TGF-α基因在SQC细胞系U-1752、两个ADC细胞系（H-23和H-125）以及3个LCC细胞系之一U-1810中均有表达。IGF-II仅在LCC细胞系U-1810中转录。除一个细胞系H-661（LCC）外，所有非小细胞癌细胞系均检测到EGF受体。在所检测的10个细胞系中均未见到IGF-I和EGF转录本。与非小细胞癌细胞系相比，4个SCC细胞系对本研究中使用的探针始终呈阴性。在所有研究的非小细胞癌而非SCC细胞系中，生长因子转录本频繁且异质性表达，这可能导致了这两种主要肺癌实体在体内和体外观察到的生物学行为差异。
