Rathbun G, Oltz E M, Alt F W
Howard Hughes Medical Institute, Boston, MA.
Int Immunol. 1993 Aug;5(8):997-1000. doi: 10.1093/intimm/5.8.997.
Analyses of mechanisms that regulate V(D)J recombination have relied heavily on the use of transformed precursor lymphocyte cell lines. We now show that such lines have highly variable and frequently low levels of recombination activating genes (RAG)-1 and -2 gene expression. We also show that expression levels of the RAG genes can vary > 100-fold between different subcloned cells of an individual pre-B line. We discuss these findings in the context of normal regulation of RAG gene expression and the implication for the use of transformed pre-B cell lines as models for studying control of V(D)J recombination activity.
对调节V(D)J重组机制的分析在很大程度上依赖于转化前体淋巴细胞系的使用。我们现在表明，这些细胞系具有高度可变且常常较低水平的重组激活基因（RAG）-1和-2基因表达。我们还表明，在单个前B细胞系的不同亚克隆细胞之间，RAG基因的表达水平可以相差100倍以上。我们在RAG基因表达的正常调节背景下讨论这些发现，以及将转化的前B细胞系用作研究V(D)J重组活性控制模型的意义。
