采用聚合酶链反应和地高辛标记的寡核苷酸探针，对德国系统性红斑狼疮患者进行HLA - DPB1等位基因的DNA分型。系统性红斑狼疮研究组成员。

DNA typing for HLA-DPB1-alleles in German patients with systemic lupus erythematosus using the polymerase chain reaction and DIG-ddUTP-labelled oligonucleotide probes. Members of SLE Study Group.

作者信息

Yao Z, Hartung K, Deicher H G, Brünnler G, Bettinotti M P, Keller E, Paul C, Gawron C, Mikschl S, Albert E

机构信息

Labor für Immungenetik, Kinderpoliklinik der Universität, München, Germany.

出版信息

Eur J Immunogenet. 1993 Aug;20(4):259-66. doi: 10.1111/j.1744-313x.1993.tb00141.x.

DOI:10.1111/j.1744-313x.1993.tb00141.x

PMID:8399121

Abstract

Genomic DNA of 178 German Caucasian patients with systemic lupus erythematosus are studied for HLA-DP locus by using PCR and DIG-ddUTP-labelled oligonucleotide probes. A significant increase of DPB10101 is observed in SLE patients compared with healthy controls (chi 2 = 15.27, p.c. < 0.004). DPB10501 and 0901 are also slightly increased (chi 2 = 5.85, P < 0.05, p.c. = NS; chi 2 = 5.64, P < 0.05, p.c. = NS). There is no significant difference in frequency of DP alleles between male and female patients. Since a linkage disequilibrium between HLA-B, DR and DP loci is found in our SLE patients, an analysis is performed assessing the relative importance of these HLA-markers to SLE. The results show that the increase of DPB10101 in SLE patients is associated with the HLA-B8, DR3 haplotype and it suggests a more important role for HLA-B8, DR3 or genes within this haplotype than for DPB1*0101 in the genetic predisposition for SLE.

摘要

采用聚合酶链反应（PCR）和地高辛标记的寡核苷酸探针，对178例德国白种人系统性红斑狼疮患者的基因组DNA进行HLA - DP位点研究。与健康对照相比，系统性红斑狼疮患者中DPB10101显著增加（卡方检验=15.27，校正P值<0.004）。DPB10501和0901也略有增加（卡方检验=5.85，P<0.05，校正P值无统计学意义；卡方检验=5.64，P<0.05，校正P值无统计学意义）。男性和女性患者之间DP等位基因频率无显著差异。由于在我们的系统性红斑狼疮患者中发现HLA - B、DR和DP位点之间存在连锁不平衡，因此进行了一项分析，以评估这些HLA标记物对系统性红斑狼疮的相对重要性。结果表明，系统性红斑狼疮患者中DPB10101的增加与HLA - B8、DR3单倍型相关，这表明在系统性红斑狼疮的遗传易感性中，HLA - B8、DR3或该单倍型内的基因比DPB1*0101起更重要的作用。