Yao Z, Hartung K, Ehrfeld H, Seelig H P, Deicher H G, Brünnler G, Keller E, Albert E
Labor für Immungenetik, Kinderpoliklinik der Universität München, Germany.
Rheumatol Int. 1993;13(4):155-8. doi: 10.1007/BF00301263.
We investigated the association of HLA-DPB1 alleles with the occurrence of autoantibodies against Ro (SS-A) or La (SS-B) using recombinant 52 kD-Ro, 60 kD-Ro and La proteins in 177 German patients with systemic lupus erythematosus (SLE). A significant increase in the frequency of DPB10101 is observed in SLE patients compared to healthy controls (Pcorr.. < 0.004). Antibodies against 52 kD-Ro, 60 kD-Ro and La are tested by ELISA and are found with a frequency of 25.4%, 33.9% and 17.5% in the patients, respectively. An association with HLA-DPB10101 is observed for antibodies against La (P < 0.01) and 52 kD-Ro (P < 0.01), but not for 60 kD-Ro in the absence of La/52 kD-Ro. Since there is a strong linkage disequilibrium between DPB10101 and DR3 in the normal population and in SLE patients, and since there is an association between DR3 and SLE, as well as between DR3 and the occurrence of recombinant Ro/La antibodies in SLE patients, we investigated whether DPB10101 is associated per se or via linkage disequilibrium with DR3. DPB10101 in the absence of DR3 is not more common in patients than in controls and not in patients with autoantibodies to Ro and La than without autoantibodies. We conclude that there is no evidence for a direct involvement of DPB10101 in the production of Ro/La autoantibodies in SLE patients.
我们使用重组的52kD - Ro、60kD - Ro和La蛋白，对177例德国系统性红斑狼疮（SLE）患者进行研究，以探究HLA - DPB1等位基因与抗Ro（SS - A）或La（SS - B）自身抗体产生之间的关联。与健康对照相比，SLE患者中DPB10101的频率显著增加（校正P值<0.004）。通过酶联免疫吸附测定法（ELISA）检测抗52kD - Ro、60kD - Ro和La的抗体，在患者中的检出频率分别为25.4%、33.9%和17.5%。观察到抗La抗体（P < 0.01）和抗52kD - Ro抗体（P < 0.01）与HLA - DPB10101有关联，但在不存在La/52kD - Ro的情况下，抗60kD - Ro抗体与HLA - DPB10101无关联。由于在正常人群和SLE患者中，DPB10101与DR3之间存在强连锁不平衡，且DR3与SLE以及SLE患者中重组Ro/La抗体的产生之间存在关联，我们研究了DPB10101是本身存在关联还是通过与DR3的连锁不平衡存在关联。在不存在DR3的情况下，DPB10101在患者中并不比在对照中更常见，在有抗Ro和La自身抗体的患者中也不比没有自身抗体的患者更常见。我们得出结论，没有证据表明DPB1*0101直接参与SLE患者中Ro/La自身抗体的产生。
