Ouhtit A, Morel G, Kelly P A
CNRS URA 1454, Neuroendocrinologie, Faculté de Médecine Lyon-Sud, Oullins, France.
Biol Reprod. 1993 Sep;49(3):528-36. doi: 10.1095/biolreprod49.3.528.
Prolactin receptor gene expression was visualized in various tissues by in situ hybridization. Probes specific to the intracellular domains of the short and long form of receptor were prepared. The specificity of these signals was controlled by competition with excess unlabeled homologous probes or heterologous probes; moreover, some tissues, such as penis and vagina, show no expression of either form of receptor mRNA. Macroautoradiogram signals (optical density) were quantified and expressed in arbitrary units. The long form of receptor mRNA was preferentially expressed in testis, epididymis, prostate, seminal vesicle, and mammary gland from lactating animals, whereas the expression of the two forms of mRNA was equivalent in ovary, uterus, and pregnant mammary gland. Signals were also localized at the light microscopic level to individual cells. This approach has permitted the precise localization of prolactin receptor mRNAs in reproductive tissues. Actions of prolactin have not been demonstrated in all tissues expressing receptor transcripts; thus it will be interesting to correlate the expression of long and short forms of receptor with specific functions.
通过原位杂交在各种组织中观察催乳素受体基因的表达。制备了针对受体短形式和长形式细胞内结构域的特异性探针。这些信号的特异性通过与过量未标记的同源探针或异源探针竞争来控制；此外，一些组织，如阴茎和阴道，未显示任何一种形式的受体mRNA表达。对宏观放射自显影片信号（光密度）进行定量，并以任意单位表示。受体mRNA的长形式在泌乳动物的睾丸、附睾、前列腺、精囊和乳腺中优先表达，而两种形式的mRNA在卵巢、子宫和妊娠乳腺中的表达相当。信号在光学显微镜水平上也定位于单个细胞。这种方法使得催乳素受体mRNA在生殖组织中的精确定位成为可能。催乳素的作用尚未在所有表达受体转录本的组织中得到证实；因此，将受体长形式和短形式的表达与特定功能相关联将会很有趣。
