Blandino A, Macías M, Cantero D
Biological and Enzymatic Reactors Research Group, Department of Chemical Engineering, Food Technology and Environmental Technologies, Faculty of Sciences, University of Cádiz, 11510 Puerto Real (Cádiz), Spain.
Appl Biochem Biotechnol. 2003 Jul;110(1):53-60. doi: 10.1385/abab:110:1:53.
Encapsulation within calcium alginate gel capsules was used to produce a coimmobilized enzyme system. Glucose oxidase (GOD) and catalase (CAT) were chosen as model enzymes. The same values of Vmax and Km app for the GOD encapsulated system and for the GOD-CAT coencapsulated system were calculated. When gel beads and capsules were compared, the same catalyst deactivation sequence for the two enzymes was observed. However, when capsules were employed as immobilization support, GOD efficiencies were higher than for the gel beads. These results were explained in terms of the structure of the capsules.
将其包封在海藻酸钙凝胶胶囊中用于制备共固定化酶系统。选择葡萄糖氧化酶（GOD）和过氧化氢酶（CAT）作为模型酶。计算了包封GOD的系统和共包封GOD-CAT的系统的Vmax和Km app的相同值。当比较凝胶珠和胶囊时，观察到两种酶具有相同的催化剂失活顺序。然而，当使用胶囊作为固定化载体时，GOD的效率高于凝胶珠。这些结果根据胶囊的结构进行了解释。
