Microanalytical studies of metal localization in biological tissues by environmental SEM.

The presence and distribution of Al and Mn in floral and seed tissues of eucalypts from Al-contaminated soils was analyzed using energy-dispersive X-ray microanalysis (EDS) in an environmental scanning electron microscope (ESEM). EDS by ESEM determined the distribution of elements between tissue types was suitable for intact samples or those with lower available moisture or intact specimens. The analytical technique was not appropriate for highly vascular samples. Other factors influencing the detection of elements by ESEM-EDS were electron scattering and the relative concentration and localization of elements within the tissues. EDS-detectable levels were significantly correlated with tissue concentrations determined by atomic absorption spectrophotometry for Mn but not for Al.