Rooney P S, Clarke P A, Gifford K A, Robinson M H, Hardcastle J D, Durrant L G, Armitage N C
Department of Surgery, University Hospital, Queen's Medical Centre, Nottingham, UK.
Eur J Cancer Prev. 1993 Sep;2(5):387-92. doi: 10.1097/00008469-199309000-00005.
The in vitro metaphase arrest technique (crypt cell production rate-CPPR) has been used to measure human rectal mucosal proliferation. Study of preincubation times, dose response curves and lag phases suggest that a concentration of vincristine of 5 micrograms/ml and 16 hour preincubation with time point increments between 25 and 125 minutes give optimal conditions for measuring rectal mucosal proliferation. Twenty individuals had rectal CCPR repeated without intervention of any kind. Close correlation was found between the two values (r = 0.89 and P = 0.0001). The effect of polyethylene glycol bowel preparation was also studied in 35 subjects. There was good correlation (r = 0.66, P = 0.007). There was close correlation between rectal and caecal CCPR as measured in 20 patients who had colonoscopy (r = 0.72, P = 0.0003). The in vitro metaphase arrest technique is a useful parameter of rectal mucosal proliferation and may be used with confidence in a number of different clinical situations.
体外中期阻断技术（隐窝细胞产生率-CPPR）已被用于测量人类直肠黏膜增殖。对预孵育时间、剂量反应曲线和延迟期的研究表明，长春新碱浓度为5微克/毫升，预孵育16小时，时间点增量在25至125分钟之间，可为测量直肠黏膜增殖提供最佳条件。20名个体在未进行任何干预的情况下重复进行直肠CCPR测量。发现两个值之间存在密切相关性（r = 0.89，P = 0.0001）。还对35名受试者研究了聚乙二醇肠道准备的效果。存在良好的相关性（r = 0.66，P = 0.007）。在20名接受结肠镜检查的患者中测量的直肠和盲肠CCPR之间存在密切相关性（r = 0.72，P = 0.0003）。体外中期阻断技术是直肠黏膜增殖的一个有用参数，可在多种不同临床情况下可靠使用。
