Iwasaki K, Makino T, Maruyama T, Matsubayashi H, Nozawa S, Yokokura T
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Japan.
Int J Fertil Menopausal Stud. 1993 Jul-Aug;38(4):229-34.
To evaluate cell-mediated immunity in endometriosis.
Peritoneal fluid and peripheral blood samples from 19 women with macroscopic evidence of endometriosis and 26 controls were submitted to 2-color flow cytometric analysis to assess lymphocyte subsets, using various combinations of monoclonal antibodies. K562 cells were used in a routine assay by 51Cr release to measure the cytotoxicity of NK cells in the subjects' peripheral blood.
Suppressor T-cells were significantly increased and cytotoxic T-cells significantly decreased in both peritoneal fluid and peripheral blood of the women with endometriosis. NK activity in peripheral blood was decreased in endometriosis patients as compared with controls.
An alteration in cell-mediated immunity may be among the pathogenetic, or developing, factors in endometriosis.
评估子宫内膜异位症中的细胞介导免疫。
收集19例有子宫内膜异位症宏观证据的女性及26例对照的腹腔液和外周血样本，使用单克隆抗体的各种组合进行双色流式细胞术分析，以评估淋巴细胞亚群。采用51Cr释放法，通过常规检测K562细胞来测定受试者外周血中自然杀伤细胞（NK细胞）的细胞毒性。
子宫内膜异位症女性的腹腔液和外周血中，抑制性T细胞显著增加，细胞毒性T细胞显著减少。与对照组相比，子宫内膜异位症患者外周血中的NK活性降低。
细胞介导免疫的改变可能是子宫内膜异位症发病机制或发展过程中的因素之一。
