Ho H N, Chao K H, Chen H F, Wu M Y, Yang Y S, Lee T Y
Department of Obstetrics and Gynaecology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Hum Reprod. 1995 Oct;10(10):2671-5. doi: 10.1093/oxfordjournals.humrep.a135765.
We examined the lymphocytes of peripheral blood (PB) and peritoneal fluid (PF) of women with or without endometriosis to investigate the alteration of cytotoxic activity of natural killer (NK) cells and activation of T cells in the peritoneal cavity of women with endometriosis. A total of 16 control women and 14 patients with stage III-IV endometriosis were selected on the basis of laparoscopic examination in National Taiwan University Hospital. The lymphocyte subpopulations (B cell, NK cell and T cell), including T-cell activation markers (CD69, CD25, HLA-DR), in PB and PF were analysed by dual-colour flow cytometry. The NK cytotoxicity of PB and PF mononuclear cells was evaluated by 51Cr release assay. There was a significant decrease of NK cytotoxicity and CD25+ CD3+ lymphocyte subpopulation in PF of women with endometriosis compared with those without endometriosis. However, there was no difference in the proportion of NK cells in both PB and PF between women with and without endometriosis. Therefore, the decreased NK cytotoxicity in PF of women with endometriosis was due to the functional defect, but not quantitative defect, of NK cells. The concomitant reduction of activated T cells in women with endometriosis might suggest its possible role in the defect of NK cytotoxicity.
我们检测了患有或未患有子宫内膜异位症女性的外周血（PB）和腹腔液（PF）中的淋巴细胞，以研究子宫内膜异位症女性腹腔中自然杀伤（NK）细胞的细胞毒性活性变化及T细胞的激活情况。基于台湾大学医院的腹腔镜检查，共选取了16名对照女性和14名III-IV期子宫内膜异位症患者。采用双色流式细胞术分析PB和PF中的淋巴细胞亚群（B细胞、NK细胞和T细胞），包括T细胞激活标志物（CD69、CD25、HLA-DR）。通过51Cr释放试验评估PB和PF单个核细胞的NK细胞毒性。与未患子宫内膜异位症的女性相比，患子宫内膜异位症女性的PF中NK细胞毒性和CD25+CD3+淋巴细胞亚群显著降低。然而，患子宫内膜异位症和未患子宫内膜异位症的女性PB和PF中NK细胞的比例并无差异。因此，子宫内膜异位症女性PF中NK细胞毒性降低是由于NK细胞的功能缺陷而非数量缺陷。子宫内膜异位症女性中活化T细胞的同时减少可能提示其在NK细胞毒性缺陷中可能发挥的作用。
